Table of Contents
What do you mean by plant tissue culture?
Plant tissue culture is defined as culturing plant seeds, organs, explants, tissues, cells, or protoplasts on a chemically defined synthetic nutrient media under sterile and controlled conditions of light, temperature, and humidity.
What is the importance of plant tissue culture in research work?
Apart from their use as a tool of research, plant tissue culture techniques have in recent years, become of major industrial importance in the area of plant propagation, disease elimination, plant improvement and production of secondary metabolites.
What are the advantages of plant tissue culture?
Advantages of Tissue Culture The new plantlets can be grown in a short amount of time. Only a small amount of initial plant tissue is required. The new plantlets and plants are more likely to be free of viruses and diseases. The process is not dependant on the seasons and can be done throughout the year.
Why sterilization is important in plant tissue culture?
Sterilization of explants is a key step in any plant tissue culture work, as the removal of all microorganisms, including bacteria and fungi, is essential to attain successful initiation, growth and development of the cultured tissues in vitro, which otherwise would be overwhelmed by the contaminants [2].
How is plant tissue culture used?
Tissue culture involves the use of small pieces of plant tissue (explants) which are cultured in a nutrient medium under sterile conditions. Using the appropriate growing conditions for each explant type, plants can be induced to rapidly produce new shoots, and, with the addition of suitable hormones new roots.
What is the importance of tissue?
A group of tissue forms an organ. The tissues are essential because it represents a level of organisation in living organisms. Tissues also function by transporting nutrients and connects bones and other body parts.
What is sterilization in plant tissue culture?
Sterilization eliminates microorganism and thus avoids contamination by bacteria and fungi. To maintain an aseptic environment, all culture vessels, media and instruments used in handling tissue, as well as the explant itself is should be surface sterilized.
What are the advantages of a plant tissue culture?
The plantlets are obtained in a very short time with a small amount of plant tissue.
What is the plant tissue culture and its applications?
Plant tissue culture is used widely in the plant sciences, forestry, and in horticulture. Applications include: The commercial production of plants used as potting, landscape, and florist subjects, which uses meristem and shoot culture to produce large numbers of identical individuals. To conserve rare or endangered plant species .
What are problems in plant tissue culture?
Recalcitrance
What are four basic types of tissues in plants?
The tissues of a plant are organized to form three types of tissue systems: the dermal tissue system, the ground tissue system, and the vascular tissue system. It consists of xylem and phloem tissues which are found as strands termed as vascular bundles.